Thermodynamics Research Center / ThermoML | Fluid Phase Equilibria

Extraction and consecutive purification of anthocyanins from grape pomace using ionic liquid solutions

Lima, A. S.[Alvaro Silva], Soares, C. M. F.[Cleide Mara Faria], Paltram, R.[Renate], Halbwirth, H.[Heidi], Bica, K.[Katharina]
Fluid Phase Equilib. 2017, 451, 68-78
ABSTRACT
The global wine industry generates approximately 8.5 million tons of waste annually, which contains several biomolecules of high value. The present study focuses on the extraction of anthocyanins from grape pomace via solid/liquid extraction with aqueous solutions of the ionic liquid 1-ethyl-3- methylimidzaolium acetate [(C2mim]OAc) and sequential purification in aqueous two-phase systems (ATPS) with additional salts. The optimum conditions for solid/liquid extraction were obtained using response surface methodology and yielded up to 3.58 mg g-1 anthocyanins. During ATPS formation, the highest purification factor (PF = 16.2 fold) was obtained in the bottom and salt-rich phase using K2CO3 (29.41 wt%) and [C2mim]OAc (29.28 wt%) at 35 C and atmospheric pressure. The partition process is exothermic, spontaneous and governed by enthalpy forces.
Compounds
# Formula Name
1 CK2O3 potassium carbonate
2 K3O4P tripotassium phosphate
3 H2O water
4 C8H14N2O2 1-ethyl-3-methylimidazolium acetate
Datasets
The table above is generated from the ThermoML associated json file (link above). POMD and RXND refer to PureOrMixture and Reaction Datasets. The compound numbers are included in properties, variables, and phases, if specificied; the numbers refer to the table of compounds on the left.
Type Compound-# Property Variable Constraint Phase Method #Points
  • POMD
  • 4
  • 1
  • 3
  • Mass fraction - 4 ; Liquid mixture 1
  • Mass fraction - 1; Liquid mixture 1
  • Pressure, kPa; Liquid mixture 1
  • Temperature, K; Liquid mixture 1
  • Liquid mixture 1
  • Liquid mixture 2
  • cloud point
  • 18
  • POMD
  • 4
  • 1
  • 3
  • Mass fraction - 1 ; Liquid mixture 1
  • Mass fraction - 4 ; Liquid mixture 2
  • Mass fraction - 1 ; Liquid mixture 2
  • Mass fraction - 4; Liquid mixture 1
  • Temperature, K; Liquid mixture 2
  • Pressure, kPa; Liquid mixture 2
  • Liquid mixture 1
  • Liquid mixture 2
  • Titration method
  • Titration method
  • Titration method
  • 3
  • POMD
  • 4
  • 2
  • 3
  • Mass fraction - 4 ; Liquid mixture 1
  • Mass fraction - 2; Liquid mixture 1
  • Pressure, kPa; Liquid mixture 1
  • Temperature, K; Liquid mixture 1
  • Liquid mixture 1
  • Liquid mixture 2
  • Titration method
  • 11
  • POMD
  • 4
  • 2
  • 3
  • Mass fraction - 2 ; Liquid mixture 1
  • Mass fraction - 4 ; Liquid mixture 2
  • Mass fraction - 2 ; Liquid mixture 2
  • Mass fraction - 4; Liquid mixture 1
  • Temperature, K; Liquid mixture 2
  • Pressure, kPa; Liquid mixture 2
  • Liquid mixture 1
  • Liquid mixture 2
  • Titration method
  • Titration method
  • Titration method
  • 4